KMID : 0363720070400040319
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Korean Journal of Anatomy 2007 Volume.40 No. 4 p.319 ~ p.328
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Voltage-dependent Calcium Channel (VDCC) ¥á1A Subunit Expression in the Ataxic Mutant, Pogo Mice Cerebellum
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Lee Nam-Seob
Kim Chul-Tae Han Seung-Yun Kim Jin-Seong Kim Jae-Min Son Jin-A Jeong Young-Gil
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Abstract
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The pogo mouse is a new ataxic mutant derived from a Korean wild mouse. The pogo mutation is inherited as an autosomal recessive trait on chromosome 8. Mutations in gene coding for the ¥á1A subunit of voltagegated P/Q-type Ca2+ channel have been shown to cause phenotypes in humans and mice, i.e., tottering, leaner, rolling mouse mouse Nagoya. Using immunohistochemistry, the expression of the ¥á1A subunit of voltage-gated P/Q-type Ca2+ channel was examined in pogo mice cerebellum including deep cerebellar nuclei (DCN). We observed ¥á1A immunoreactivity in the cerebellar cortex (Purkinje cell and granule cell) and DCN of ataxic pogo mice and heterozygote control mice. There was no difference in cerebellar cortical ¥á1A immunoreactivity between ataxic pogo mice and heterozygous littermate controls (pogo/+). However, we observed ¥á1A immunoreactivity in the Purkinje cells of control and ataxic pogo mice cerebellum and DCN. We found a significant difference between pogo and heterozygous controls in terms of ¥á1A immunoreactivities in the DCN. ¥á1A immunoreactivity in this nucleus in pogo was much higher than in heterozygous littermate controls. No significant differences were observed in the interposed nucleus between pogo and heterozygous controls, but we found that the ¥á1A subunits were clearer and more abundant in the lateral and medial regions of pogo than in control mice in these regions, where only weak immunoreactivity was observed. This elevated expression of the ¥á1A subunit in deep cerebellar neurons of pogo might be a compensation for the altered function of P/Q type calcium channel and be related with the induction of the ataxic phenotype in pogo mice.
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KEYWORD
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Voltage-dependent calcium channel (VDCC), Deep cerebellar nucleus, Pogo mice, Ataxia
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